웹IVT RNA generation: The 100 ng (in 3ul volume at most) extracted mRNA was first anealed with 1ul PolyT primer (12 uM) for 3 min at 75 ℃ and 2 min at 42 ℃. Then we prepared the first strand cDNA mix through adding 2ul 5× First-Strand buffer in SMARTScribe Reverse Transcriptase (Takara, ... 웹2024년 4월 2일 · De Westerscheldetunnel is met een lengte van ruim zes kilometer de langste boortunnel van Nederland. De tunnel verbindt Walcheren met Zeeuws-Vlaanderen en was op 14 maart jongstleden exact twintig jaar in gebruik. “We hebben een deel van de tunneltechnische installaties ondertussen vervangen”, vertelt Harald Schoenmakers, …
DNA Template Preparation for in vitro Transcription - Thermo …
웹Cap 구조와 Poly(A) 서열을 가진 mRNA를 간편하게 합성하는 all-in-one kit *; T7 RNA Polymerase를 이용한 고효율 in vitro transcription (IVT)을 위한 조성 최적화 (20 ㎕ 반응 당 … 웹2일 전 · ①TriLink社製のCapアナログ(CleanCap Reagent AGなど)を用いたin vitro transcription(IVT)に使用可能な鋳型プラスミドを簡便に構築することができるクロー … picture frames north lakes
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